Antinociceptive Antinociceptive Evaluation of Antidepressants (Cita- lopram, Duloxetine and Amitriptyline) in a Mouse Model of Acute Pain

Authors

  • Sidra Shah Department of Pharmacy, Continental Medical College, Lahore.
  • Mudassara Saqib Department of Pharmacology and Therapeutics, Shaikh Zayed Medical Complex, Lahore.
  • Azka Khan Department of Pharmacology, Rashid Latif Khan University & Medical College, Lahore.

DOI:

https://doi.org/10.47489/szmc.v38i2.462

Abstract

Introduction: Acute pain is a pervasive and debilitating symptom affecting the general population as well as depressed patients who suffer even more from pain and fatigue.

Aims and Objectives: To evaluate the antinociceptive effect induced by antidepressants (Citalopram, Duloxetine and Amitriptyline) in acute pain induced in mice.

Place and Duration of study: Animal Research Laboratory of University of Lahore. Study Period: 2018- 2019

Material and Methods: An experimental animal study was carried out on 25 albino mice. The mice were divided into 5 groups; Group 1: Positive Control (PC), Group 2: Ibuprofen (IBO) (Standard) 80mg/kg, Group3: Citalopram (CTP)3mg/kg, Group 4: Duloxetine (DXT) 25mg/kg, Group 5: Amitriptyline (AMT) 15mg/kg. Groups 2-5 were administered drugs orally through a feeding tube as per group designation while Group 1: Control was given an equivalent amount of normal saline. One hour post treatment with test drugs, all Groups (1-5) were injected 10 ml/ kg of 2% acetic acid I/P and number of writhing movements consisting of contraction of abdominal muscles leading to extension of hind limb and periodic arching of body as well as paw licking behavior were counted for 20 minutes at 5- minute interval, using hand tally counter. Degree of analgesia was calculated by using formula of percentage effectiveness. Data was entered and analyzed utilizing SPSS version 21, p?0.05 was taken as significant.

Results: Ibuprofen completely eliminated the nociceptive outcome of diluted acetic acid in writhing and licking of experimental animals. Citalopram, showed maximum decreasing effect (95%-96%) in writhing in 5-20 min. However, its effect in paw licking was low. Duloxetine showed 70% to 90% decrease in writhing at 5-20 min whereas 100 % inhibition of paw-licking was observed at 5 to 20 minutes. The writhing effect of amitriptyline was 77%-90% at 5-20 min and paw licking effect was 80-90 % at 5-20 min.

Conclusion: Amitriptyline, duloxetine and citalopram have significant effect in reducing the acute nociceptive pain and may be utilized as analgesic in acute state of pain.

References

Waitemata, D.H.B. (2017). Citalopram and escitalopram - same but different. Available from: www.saferx.co.nz/full/citalopram_escitalopra m.pdf(Accessed May, 2021)

Obata, H. (2017). Analgesic Mechanisms of Antidepressants for Neuropathic Pain. Int J Mol Sci. 18(11):2483. doi: 10.3390/ijms18112483.

Liu, Y., Zhao, J., Fan, X., Guo, W. (2019).

Dysfunction of Serotonergic and Noradrenergic Systems and Somatic Symptoms in Psychiatric Disorders. Front. Psychiatry 10:286. doi: 10.3389/fpsyt.2019.00286

Bonilla-Jaime, H., Sánchez-Salcedo, J.A., Estevez- Cabrera, M.M., Molina-Jiménez, T., Cortes- Altamirano, J.L., Alfaro-Rodríguez, A. (2022). Depression and Pain: Use of Antidepressants. CurrNeuropharmacol. 20(2):384-402. doi: 10.2174/1570159X19666210609161447.

Ciaramella, A. (2019). Psychopharmacology of chronic pain. Handbook of Clinical Neurology; Elsevier. 165:317–337.

Nicolás Lobos, Ramiro Javier Zepeda, Teresa Pelissier, Gonzalo Bustos-Quevedo, Sebastián Lux , José Luis Marcos et al (2023)Rosuvastatin Synergistically Enhances the Antinociceptive Efficacy of Duloxetine in Paclitaxel-Induced Neuropathic Pain in MiceInt. J. Mol. Sci. 2023, 24(9),8359; https://doi.org/10.3390/ijms24 098359

Palleria, C., Di Paolo, A., Giofrè, C., Caglioti, C., Leuzzi, G., Siniscalchi, A. (2013). Pharmacokinetic drug-drug interaction and their implication in clinical management. J Res Med Sci. 18(7):601-10.

Unterecker, S., Pfuhlmann, B., Kopf, J., Kittel- Schneider, S., Reif, A., Deckert, J. (2015). Increase of Heart Rate and QTc by Amitriptyline, But Not by Venlafaxine, Is Correlated to Serum Concentration. J ClinPsychopharmacol. 35(4):460-3

Onu?u, A.H. (2015). Duloxetine, an antidepressant with analgesic properties - a preliminary analysis. Rom J Anaesth Intensive Care. 22(2):123-128.

Asnis, G.M., Henderson, M.A. (2015). Levomilnacipran for the treatment of major depressive disorder: a review. Neuropsychiatr Dis Treat.11:125-35.

Yemitan, O.K., Adeyemi, O.O. (2017). Mechanistic assessment of the analgesic, anti-inflammatory and antipyretic actions of Dalbergiasaxatilis in animal models. Pharm Biol. 55(1):898-905. doi: 10.1080/13880209.2017.1283706.

Zhang TT, Xue R, Zhu L, Li J, Fan QY, Zhong BH, Li YF, Ye CY, Zhang YZ. Evaluation of the analgesic effects of ammoxetine, a novel potent serotonin and norepinephrine reuptake inhibitor. Acta Pharmacologica Sinica. 2016 Sep;37(9):1154-

Skånland, S.S., Cie?lar-Pobuda, A. (2019). Off- Label Uses of Drugs for Depression. Eur JPharmacol. 865;172732

Sarah Awad , Amira Shahid Sheikh , Dr. Mubashir Ahmad ,Dr. Fahad Abid, Zahra Mazhar, Dr. Syed Hyder Raza (2024) Antidepressant use and side effects among adolescents: an integrated review Journal of Population Therapeutics & Clinical Pharmacology 31(3), 1852–1861. https://doi.org/10.53555/jptcp.v31i3.5205

Ardid D, Marty H, Fialip J, Privat AM, Eschalier A, Lavarenne J. Comparative effects of different uptake inhibitor antidepressants in two pain tests in mice. Fundamental & clinical pharmacology. 1992 Mar;6(2):75-82.

Sawynok J, Reid AR, Esser MJ. Peripheral antinociceptive action of amitriptyline in the rat formalin test: involvement of adenosine. Pain. 1999 Mar 1;80(1-2):45-55.

Rejon-Orantes, Jose del Carmen; Sanchez-Cartela et al (2022) Extended evaluation of the acetic acid – induced writhing test in the mice Pharmacologyonline 139-146 ISSN: 1827-8620

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Published

2024-04-29

How to Cite

1.
Sidra Shah, Mudassara Saqib, Azka Khan. Antinociceptive Antinociceptive Evaluation of Antidepressants (Cita- lopram, Duloxetine and Amitriptyline) in a Mouse Model of Acute Pain. Proceedings S.Z.M.C [Internet]. 2024 Apr. 29 [cited 2024 Jun. 25];38(2):134-7. Available from: http://www.proceedings-szmc.org.pk/index.php/szmc/article/view/462