Anti-hyperuricemic Evaluation of Swertia-chirayita and Febuxostat on Potassium Oxonate Induced Hyperuricemic Animal Model

Authors

  • Qura-Tul-Ain Department of Pharmacology, Pak Red Crescent Medical & Dental College, Dina Nath Kasur.
  • Naseem Saud Ahmad Department of Pharmacology, Azra Naheed Medical College, Lahore.
  • Sidra Mushtaq Department of Pharmacology, Independent Medical College, Faisalabad.
  • Navida Manzoor Department of Pharmacology, Rashid Latif Medical College, Kasur, Lahore.
  • Akfish Zaheer Department of Pharmacology, Independent Medical College, Faisalabad.
  • Faiza Khan Department of Pharmacology, Pak Red Crescent Medical & Dental College, Dina Nath Kasur.

DOI:

https://doi.org/10.47489/szmc.v38i2.509

Keywords:

Hyperuricemia, in-vivo, Swertia chirayita, Febuxostat

Abstract

Introduction: Hyperuricemia is a metabolic disease characterized by an increase in serum uric acid levels. Hyperuricemia can be controlled by low purine diet or drugs like allopurinol and febuxostat; however, the incidence of adverse effects to these drugs, especially on chronic use, is a major clinical problem. Swertia chirayita; a herb from Gentianaceace family, was selected to observe it’s in vivo uric acid lowering effect in white Albino Wistar rats in whom hyperuricemia was induced through potassium oxonate. Febuxostat was used as a standard.

Aims and Objectives: To observe the in vivo effect of different doses of Swertia chirayita extract (SCE) on uric acid levels and to compare its effect with the allopathic medicine febuxostat.

Place and Duration of study: A randomized controlled study was held at University of Health Sciences Lahore between June- August 2017.

Material and Methods: Forty-eight healthy adult Albino Wistar rats were randomly divided into six groups. Group I and II served as negative and positive controls respectively. Hyperuricemia was induced by injecting KO solution (01ml) intraperitoneally on 1st, 3rd and 7th day of study. Group III received febuxostat 5 mg/kg through oral gavage. Group IV, V and VI were experimental groups, treated with 100, 200 and 400 mg/kg doses of Swertia Chirayita extract respectively through oral route, 02 hours after induction by KO once daily for 07 days. Blood samples were collected to record serum uric acid levels on day zero, first, third and seventh days. (SPSS)version 20 was used for data entry and analysis, pvalue ? 0.05 was given statistical importance.

Results: In all experimental groups (IV, V, VI), serum uric acid levels were significantly reduced by Swertia chirayita extract (SCE) (p ? 0.001) as compared to hyperuricemic rats (group II). SCE showed dose dependent SUA suppression. Results of both febuxostat and SCE showed that average SUA levels of these groups were markedly less than hyperuricemic rats (Group II) (p < 0.001)

Conclusion: In the light of its hypouricemic activity; we conclude that in the future, Swertia chirayita has the potential for newer therapeutic applications in this regard.

References

Raja S, Kumar A, Aahooja RD, Thakuria U, Ochani S, Shaukat F. Frequency of hyperuricemia and its risk factors in the adult population. Cureus. 2019 Mar 6;11(3).

Ali MM, Mosbah SK, Abo El-Fadl NH. Factors affecting quality of life and work productivity among patients with gout. American Journal of Nursing Research. 2019. 7(2): 128-35.

Borghi C, Agabiti-Rosei E, Johnson RJ, Kielstein JT, Lurbe E, Mancia G, Redon J, Stack AG, Tsioufis KP. Hyperuricaemia and gout in cardiovascular, metabolic and kidney disease. European journal of internal medicine. 2020 Oct 1;80:1-1.

Gliozzi M, Malara N, Muscoli S, Mollace V. The treatment of hyperuricemia. Inter. J. Cardiol. 2016; 213: 23-7.

Pandey M, Kumar V, Singh D, Kumar P, Khatoon Y, Goel V et al. Phytochemicals and evaluation of antioxidant and anti-inflammatory potentials of Swertia Chirata leaves extract in animal model. China Petroleum Processing and Petrochemical Technology. Catalyst Research. 2023; 23(2) :2508- 18.

Afzal A, Aftab B, Siddique J, Babar S, Sohail A, Yasir M, Hanif S. Phytochemical and antimicrobial activity analysis of Swertia chirayita and Artemisia absinthium plant extracts. Biological and Clinical Sciences Research Journal. 2021 Aug 24;2021(1).

Haidari F, Rashidi MR, Keshavarz SA, Mahboob SA, Eshraghian MR, Shahi MM. Effects of onion on serum uric acid levels and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats. Pak. J. Biol. Sci. 2008; 11(14): 1779-84.

Manjunatha KP. Antidiabetic efficacy of Swertia chirayita extract in streptozotocin induced diabetic wistar rats Group. 2023;3(15):30.

Shi H, Liang XS, Huang LW, Luo ZG, Tan L. The optimization and assessment of the method for inducing hyperuricemia in rats. Zhongguo ying yong sheng li xue za zhi= Zhongguo yingyong shenglixue zazhi= Chinese journal of applied physiology. 2020 May 1;36(3):223-7.

Wang WL, Sheu SY, Huang WD, Chuang YL, Tseng HC, Hwang TS, et al. Phytochemicals from Tradescantia albiflora Kunth extracts reduce serum uric acid levels in oxonate- induced rats. Pharmacogn. Mag. 2016; 12: 223- 7.

Sato VH, Sungthong B, Rinthong PO, Nuamnaichati N , Mangmool S, Chewchida S et l.Pharmacological effects of Chatuphalatika in hyperuricemia ofgout. Pharma. Biol. 2018; 56(1): 76-85.

Haidari F, Rashidi MR, Keshavarz SA, Mahboob SA, Eshraghia MR, Shahi MM. Effects of onion on serum uric acid levels and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats. Pak. J. Biol. Sci. 2008; 11(14): 1779-84.

Nandipati MC, Sumalatha G, Baburao C, Babu JR, Sridevi C. Antitumor activity of mimosa rubicaulis lam against ehrlich ascites carcinoma in Swiss albino mice. I. J. P. S. R. 2014; 5(4): 1514-24.

Lin H, Geng S, Yang L, Yang L, Qi M, Dong B, Xu L, Wang Y, Lv W. The effect of metabolic factors on the association between hyperuricemia and chronic kidney disease: a retrospective cohort mediation analysis. International Urology and Nephrology. 2024 Feb 21:1-1.

Song P, Wang H, Xia W, Chang X, Wang M, An L. Prevalence and correlates of hyperuricemia in the middle-aged and older adults in China. Sci. Rep.2018; 8(1): 1-9.

Robinson PC, Dalbeth N. (2018): Febuxostat for the treatment of hyperuricaemia in gout, Expert Opinion on Pharmacotherapy. ISSN: 1465-6566 (Print) 1744-7666 (Online) Journal homepage: http://www.tandfonline.com/loi/ieop20. DOI: 0.1080/14656566.2018.1498842

Hu M, Tomlinson B. Febuxostat in the management of hyperuricemia and chronic gout: a review. Ther. Clin. Risk. Manag. 2018; 4(6): 1209-20.

Akbar S. Swertia chirata Buck -Ham. Ex Wall. Swertia chirayita (Roxb.) H. Karsten (Gentianaceae). (2020). In: Handbook of 200 Medicinal Plants. Springer, Cham. https://doi.org/10.1007/978-3-030-16807-0_176

Fatema-Tuz-Zohra, Rashid F, Jashim T, Cruze LRM, Das R, Afrin N. An in vivo assessment of anti-diabetic effect of Swertia Chirata on alloxan induced diabetes rat and assess the safety profile. World J. Pharm. Res. 2019; 8(13): 329-40.

Roy, S. (2019-2020). Phytochemical screening and antimicrobial efficacy of Ocimum sanctum (Tulsi) and Swertia chirayita (Chirota) against Escherichia coli and Salmonella spp. isolated from poultry and their molecular study. [Master of Science in Biochemistry. Faculty of Veterinary Medicine Chattogram Veterinary and Animal Sciences University Chattogra, Bangladesh]

Mehmood A, Ishaq M, Zhao L, Safdar B, Rehman A, Munir M, et al. Natural compounds with xanthine oxidase inhibitory activity: A Review. Chem. Biol. Drug Des. 2019; 93 (4): 387-418.

Ziyaurrahman AR, Kale P, Narkhede S. Int. J. Adv. Pharmacy. Med. Bioallied. Sci. 2017: 1-5

Downloads

Published

2024-04-29

How to Cite

1.
Qura-Tul-Ain, Naseem Saud Ahmad, Sidra Mushtaq, Navida Manzoor, Akfish Zaheer, Faiza Khan. Anti-hyperuricemic Evaluation of Swertia-chirayita and Febuxostat on Potassium Oxonate Induced Hyperuricemic Animal Model. Proceedings S.Z.M.C [Internet]. 2024 Apr. 29 [cited 2024 Dec. 9];38(2):144-9. Available from: http://www.proceedings-szmc.org.pk/index.php/szmc/article/view/509